Cell Analysis

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ANNEALING AND GALVANIZING FURNACES

A continuous galvanizing line (CGL) restores the mechanical properties of the cold rolled steel by heating the strip up to temperature then applying a metallic coating to it’s surface by dipping into a molten bath of Zinc/Aluminum. This improves the corrosion resistance of the steel strip. Using image analysis with the CANTY Ultratemp Camera and Intelligent machine learning software, the position of the strip on the rollers can be tracked to prevent process upsets such as strip tearing while also allowing for visual verification form the operator. Control signals are output to the DCS via 4-20mA, Modbus TCP/IP or OPC UA.

A continuous annealing line (CAL) is a specialized industrial process for heat-treating steel strip, modifying its microstructure and properties like hardness and ductility, often to improve its formability. It involves passing the steel strip through a furnace where it’s heated, soaked, and cooled under a controlled atmosphere.

CANTY High Temperature Cameras are ideal for demanding applications involving visual inspection or verification in extreme temperature environments. CANTY High Temperature Camera Systems feature a fused glass seal standard equipment with every model. This unique seal provides an impenetrable safety barrier to protect the camera electronics from the harsh process environment and preventing hazardous vapors from escaping into your plant.

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BATCH

Batch cell culture is a closed system where cells grow in a finite volume of liquid medium with an initial set of nutrients. The culture progresses through distinct phases: lag, exponential, stationary, and death, before being harvested. CANTY cell analysis in this method is crucial for understanding cell viability and identifying when cells are undergoing apoptosis and experiencing other stresses, especially as nutrients deplete and waste products accumulate.

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BIOFILM

In pharmaceutical water systems, particularly WFI (Water for Injection) loops, biofilms pose a significant risk of contamination. Biofilms are essentially communities of microorganisms that attach to surfaces and create a protective matrix, making them difficult to remove and potentially leading to issues with product quality and safety. The CANTY INFLOW™ monitors water quality through particle analysis which is necessary to identify those microorganisms and address potential issues early on.

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MEDIA PREP DILUTION

Media preparation refers to the process of creating a nutrient-rich solution, or culture medium, that supports the growth and propagation of microorganisms, cells, or tissues in a laboratory setting. Carefully combining specific ingredients, such as nutrients, buffering agents, and growth factors, to provide the optimal environment for the target biological material.

Dilution is the process of making a solution less concentrated by adding more solvent (usually water). It’s a crucial step for various reasons, including isolating pure cultures, achieving desired concentrations for testing, and minimizing interference from other components in a sample.

CANTY gives you the tools for real time analysis of the dilution process for volumetric inaccuracies, contamination, and inconsistencies.

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PARTICLES IN WATER

WFI is a high-purity water used as a solvent or diluent for injectable medications. It is produced to very stringent quality standards, with particulate matter being a key quality attribute. WFI must be essentially free of visible particles and have very low levels of sub-visible particles. CANTY’s AI-trained software analyzes these factors to recognize patterns specific to each particle type, distinguishing between different types of particles within the same analysis and to accurately quantify measurements for each class individually.

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PERFUSION

Perfusion Cell Culture is a biotechnological process carried out over an extended period of time in which cells are grown and harvested while the media and nutrients they use is constantly replenished. Throughout this process it is vital to measure the count and viability of cells in a given volume to ensure the culture has maintained its target for growth and production. Analyzing these cells continuously produces cell count and viability data which can better inform decisions for process control methods. CANTY dynamic imaging technology measures cell count and viability in real time as the outlet of a perfusion culture passes through an at-line flow cell.

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ROUGING

Rouging in WFI (Water for Injection) systems refers to the formation of reddish-brown to dark violet iron deposits on the interior surfaces of stainless steel equipment, particularly in pharmaceutical and biotech industries. This phenomenon is a type of corrosion and is primarily caused by the oxidation of iron in the stainless steel, especially in the presence of hot WFI (above 60°C). Rouge can detach from the surfaces and contaminate the WFI, potentially affecting the quality and safety of pharmaceutical products. The CANTY InFlow™ detects these particles in real time, quantifying the amount of rouge being generated, providing an objective measure of system performance.

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SINGLE USE PERFUSION

Single-use perfusion involves using disposable bioreactors for continuous cell culture processes, where fresh medium is constantly supplied while waste is removed, allowing for high cell densities and product yields. This approach combines the benefits of perfusion culture with the advantages of single-use technology, such as reduced risk of contamination and faster setup times. CANTY Single Use Analyzer has been engineered to analyze small volume samples for particle size, shape and concentration in single use systems to ensure high productivity and improved product quality.

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CELL ANALYSIS

The Cell Analysis system is able to determine the condition of a cell by its appearance. This enables real time analysis without having to employ additive reagents. The results are comparable to Trypan Blue assays. See detailed information in the Data Sheet, Application Brochure and presentations in the Product Documents tab below.

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